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Global gene expression in channel catfish after vaccination with an attenuated Edwardsiella ictaluri
- Prigeon, Julia W., Yeh, Hung-Yueh, Shoemaker, Craig A., Mu, Xingjiang, Klesius, Phillip H.
- Fish and Shellfish Immunology 2012 v.32 pp. 524
- Edwardsiella ictaluri, Ictalurus punctatus, apolipoprotein A-I, apoptosis, cell structures, fish, gene expression, gene expression regulation, genes, immune response, live vaccines, messenger RNA, microarray technology, polymerase chain reaction, protein synthesis, signal transduction, transcription (genetics), translation (genetics), vaccination
- To understand the global gene expression in channel catfish after immersion vaccination with an attenuated Edwardsiella ictaluri (AquaVac-ESC), microarray analysis of 65,182 UniGene transcripts was performed. With a filter of false-discovery rate less than 0.05 and fold change greater than 2, a total of 52 unique transcripts were found to be upregulated in vaccinated fish at 48 h post vaccination, whereas a total of 129 were downregulated. The 52 upregulated transcripts represent genes with putative functions in the following seven major categories: (1) hypothetical (25%); (2) novel (23%); (3) immune response (17%); (4) signal transduction (15%); (5) cell structure (8%); (6) metabolism (4%); and (7) others (8%). The 129 downregulated transcripts represent genes with putative functions in the following ten major categories: (1) novel (25%); (2) immune response (23%); (3) hypothetical (12%); (4) metabolism (10%); (5) signal transduction (7%); (6) protein synthesis (6.2%); (7) cell structure (5%); (8) apoptosis (3%); (9) transcription/translation (2%); and (10) others (6%). Microarray analysis revealed that apolipoprotein A-I was upregulated the most (8.5 fold, P ¼ 0.011) at 48 h post vaccination whereas a novel protein (accession no. CV995854) was downregulated the most (342 fold, P ¼ 0.001). Differential regulation of several randomly selected transcripts in vaccinated fish was also validated by quantitative PCR. Our results suggest that these differentially regulated genes elicited by the vaccination might play important roles in the protection of channel catfish against E. ictaluri.