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Identification and anti-tumour activities of phenolic compounds isolated from defatted adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seed meal

Author:
Chen, Chao, Zhang, Yiyi, Gao, Yulong, Xu, Qian, Ju, Xingrong, Wang, Lifeng
Source:
Journal of functional foods 2016 v.26 pp. 394-405
ISSN:
1756-4646
Subject:
Coix lacryma-jobi, Western blotting, acetone, antineoplastic activity, butanol, caspase-3, caspase-9, cytochrome c, electrospray ionization mass spectrometry, ferulic acid, inhibitory concentration 50, neoplasms, nuclear magnetic resonance spectroscopy, p-coumaric acid, rutin
Abstract:
In this work, four phenolic compounds, N1,N5-di-[(E)-p-coumaroyl]-spermidine (1), p-coumaric acid (2), ferulic acid (3), and rutin (4) were isolated from n-butanol fraction of acetone extract of defatted adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) seed meal. Compound 1 was identified from adlay for the first time. The structures of these compounds were characterised by ESI-MS, 1H NMR, 13C NMR and UV–vis techniques. These compounds were evaluated for their anti-tumour activities against HepG-2, MCF-7 and CaCo-2 cancer cell lines by MTT assay. Of all purified compounds, compound 1 possessed the highest anti-tumour activity than other compounds with IC50 values between 46.34 ± 3.99 and 92.69 ± 5.63 µg/ml. Furthermore, activation of the caspase cascade in HepG-2 cells for intrinsic pathways caused by compound 1 were observed using western blot analysis. The result also showed that the expression of caspase-3, caspase-9, cytochrome c and Bax protein increased and the protein level of Bcl-2 decreased.
Agid:
5546322