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Organochlorinated pesticide degrading microorganisms isolated from contaminated soil

Lovecka, Petra, Pacovska, Iva, Stursa, Petr, Vrchotova, Blanka, Kochankova, Lucie, Demnerova, Katerina
New biotechnology 2015 v.32 pp. 26-31
DDD (pesticide), DDE (pesticide), DDT (pesticide), DNA, HCH (pesticide), bioremediation, gas chromatography, genes, hexachlorobenzene, lindane, polluted soils, polychlorinated biphenyls, ribosomal RNA, sequence analysis, soil microorganisms, Czech Republic
Degradation of selected organochlorinated pesticides (γ-hexachlorocyclohexane – γ-HCH, dichlorodiphenyltrichloroethane – DDT, hexachlorobenzene – HCB) by soil microorganisms was studied. Bacterial strains isolated from contaminated soil from Klatovy-Luby, Hajek and Neratovice, Czech Republic, capable of growth on the selected pesticides were isolated and characterised. These isolates were subjected to characterisation and identification by MS MALDI-TOF of whole cells and sequence analysis of 16S rRNA genes. The isolates were screened by gas chromatography for their ability to degrade the selected pesticides. Some isolates were able to degrade pesticides, and the formation of degradation products (γ-pentachlorocyclohexane (γ-PCCH), dichlorodiphenyldichloroethylene (DDE) and dichlorodiphenyldichloroethane (DDD)) observed in liquid culture confirmed their degradation capability. The isolates and DNA samples isolated from the contaminated soil were also screened for the bphA1 gene (encoding biphenyl-2,3-dioxygenase, the first enzyme in the PCB degradation pathway) and its occurrence was demonstrated. The isolates were also screened for the presence of linA, encoding dehydrochlorinase, the first enzyme of the HCH degradation pathway. The linA gene could not be found in any of the tested isolates, possibly due to the high specificity of the primers used. The isolates with the most effective degradation abilities could be used for further in situ bioremediation experiments with contaminated soil.