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Proteomic study on the effects of silver nanoparticles on soybean under flooding stress
- Mustafa, Ghazala, Sakata, Katsumi, Hossain, Zahed, Komatsu, Setsuko
- Journal of proteomics 2015 v.122 pp. 100-118
- Glycine max, alcohol dehydrogenase, biochemical pathways, byproducts, cluster analysis, cytotoxicity, fermentation, floods, gene expression regulation, genes, glycolysis, growth performance, nanosilver, proteins, proteomics, pyruvate decarboxylase, roots, seedling growth, soybeans, transcription (genetics)
- Flooding negatively affects the soybean growth; however, silver nanoparticles (AgNPs) enhanced the growth under stress. To study the effects of AgNPs on soybean under flooding, a gel-free proteomic technique was used. The morphological analysis of early-stage soybean exposed to flooding with AgNPs of various sizes and concentrations revealed enhanced seedling growth by treatment with 15nm AgNPs at 2ppm. Differentially changed 107 root proteins were predominantly associated with stress, signaling, and cell metabolism. Hierarchical clustering divided these proteins into 3 clusters. Based on cluster analysis, the abundances of glyoxalase II 3 and fermentation related proteins were time-dependently increased under flooding stress, but decreased in response to AgNPs. Six enzymes involved in metabolic pathways were analyzed at the transcriptional level. The alcohol dehydrogenase 1 and pyruvate decarboxylase 2 genes were up-regulated under flooding stress while down-regulated in response to AgNPs. Moreover, comparatively low transcript level of glyoxalase II 3 under AgNPs treatment implies that less cytotoxic by-products of glycolysis are produced in AgNPs exposed soybeans as compared to flooded soybean. These results suggest that the AgNPs treated soybeans might have experienced less oxygen-deprivation stress, which might be the key factor for better growth performance of AgNPs treated soybeans under flooding stress.This study highlighted the effect of silver nanoparticles (AgNPs) on the soybean under flooding stress. Silver nanoparticles (2ppm AgNPs, 15nm in size) treatment facilitate the soybean under flooding stress enhancing seedling growth. A time-course comparative gel-free proteomic study was performed to analyze the changes inproteome profiles in response to AgNPs treatment under flooding. The 107 differentially changed root proteins were predominantly associated with stress, signaling, cell metabolism. The abundances of the glyoxalase II 3 and fermentation related proteins were significantly increased on exposure to flooding; however, decreased by AgNPs treatment. Comparatively low transcript level of glyoxalase II 3 under AgNPs treatment implies that less cytotoxic by-products of glycolysis are produced in AgNPs exposed soybeans as compared to flooded soybean. Moreover, the observed up-regulation of the alcohol dehydrogenase 1 and pyruvate decarboxylase 2 genes under flooding stress condition and its down-regulation in response to AgNPs treatment might be related to a metabolic shift towards normal cellular processes.