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T7 Peptide-Functionalized PEG-PLGA Micelles Loaded with Carmustine for Targeting Therapy of Glioma

Author:
Bi, Yunke, Liu, Lisha, Lu, Yifei, Sun, Tao, Shen, Chen, Chen, Xinli, Chen, Qinjun, An, Sai, He, Xi, Ruan, Chunhui, Wu, Yinhao, Zhang, Yujie, Guo, Qin, Zheng, Zhixing, Liu, Yaohua, Lou, Meiqing, Zhao, Shiguang, Jiang, Chen
Source:
ACS Applied Materials & Interfaces 2016 v.8 no.41 pp. 27465-27473
ISSN:
1944-8252
Subject:
biocompatibility, biodegradability, blood-brain barrier, body weight, brain neoplasms, central nervous system, clinical trials, drugs, evaporation, excision, light scattering, micelles, nanoparticles, transmission electron microscopy
Abstract:
Glioma is regarded as the deadliest and most common brain tumor because of the extremely difficult surgical excision ascribed from its invasive nature. In addition, the natural blood-brain barrier (BBB) greatly restricts the therapeutics’ penetration into the central nervous system. Carmustine (BCNU) is a widely used antiglioma drug in clinical applications. However, its serious complications prevent it from being applied in a clinical setting to some extent. Thus, it is urgent to explore novel BCNU delivery systems specially designed for glioma. Development of polymeric nanoparticles offers a favorable alternative to serve this purpose. Particularly, use of poly(lactic-co-glycolic acid) (PLGA) has been shown to be advantageous for its favorable biodegradability and biocompatibility, which ensure safe therapies. In this study, T7 peptide-conjugated, BCNU-loaded micelles were constructed successfully via the emulsion-solvent evaporation method. The micelles were characterized by transmission electron microscopy and dynamic light scattering in detail, and the capacity of BBB crossing was studied. The in vivo detecting results of the targeting effect using the BODIPY probe evidenced that T7-modified micelles showed a more pronounced accumulation and accumulated in the tumor more efficiently than in the unconjugated probe. Meanwhile, the targeting group exhibited the best curative effect accompanied with the lowest loss in body weight, the smallest tumor size, and an obviously prolonged survival time among the groups. In the near future, we believe the targeted delivery system specially designed for BCNU is expected to provide sufficient evidence to proceed to clinical trials.
Agid:
5549012