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Genome-wide analysis of HOXC9-induced neuronal differentiation of neuroblastoma cells
- Wang, Xiangwei, Yang, Liqun, Choi, Jeong-Hyeon, Kitamura, Eiko, Chang, Chang-Sheng, Ding, Jane, Lee, Eun J., Cui, Hongjuan, Ding, Han-Fei
- Genomics Data 2014 v.2 pp. 50-52
- chromatin, gene expression regulation, high-throughput nucleotide sequencing, homeodomain proteins, microarray technology, neoplasms, neurons, precipitin tests, quality control, sympathetic nervous system
- Induction of differentiation is a therapeutic strategy in neuroblastoma, a common pediatric cancer of the sympathetic nervous system. The homeobox protein HOXC9 is a key regulator of neuroblastoma differentiation. To gain a molecular understanding of the function of HOXC9 in promoting differentiation of neuroblastoma cells, we conducted a genome-wide analysis of the HOXC9-induced differentiation program by microarray gene expression profiling and chromatin immunoprecipitation in combination with massively parallel sequencing (ChIP-seq). Here we describe in detail the experimental system, methods, and quality control for the generation of the microarray and ChIP-seq data associated with our recent publication .