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Bacterial tag encoded FLX titanium amplicon pyrosequencing (bTEFAP) based assessment of prokaryotic diversity in metagenome of Lonar soda lake, India

Author:
Dudhagara, Pravin, Ghelani, Anjana, Patel, Rajesh, Chaudhari, Rajesh, Bhatt, Shreyas
Source:
Genomics Data 2015 v.4 pp. 8-11
ISSN:
2213-5960
Subject:
Coxiella burnetii, Fibrobacter intestinalis, Methanoculleus, Methanosaeta, bacteria, databases, lakes, metagenomics, prokaryotic cells, sediments, sequence analysis, India
Abstract:
Bacterial diversity and archaeal diversity in metagenome of the Lonar soda lake sediment were assessed by bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP). Metagenome comprised 5093 sequences with 2,531,282bp and 53±2% G+C content. Metagenome sequence data are available at NCBI under the Bioproject database with accession no. PRJNA218849. Metagenome sequence represented the presence of 83.1% bacterial and 10.5% archaeal origin. A total of 14 different bacteria demonstrating 57 species were recorded with dominating species like Coxiella burnetii (17%), Fibrobacter intestinalis (12%) and Candidatus Cloacamonas acidaminovorans (11%). Occurrence of two archaeal phyla representing 24 species, among them Methanosaeta harundinacea (35%), Methanoculleus chikugoensis (12%) and Methanolinea tarda (11%) were dominating species. Significant presence of 11% sequences as an unclassified indicated the possibilities for unknown novel prokaryotes from the metagenome.
Agid:
5550709