Main content area

Influence of nutrient media on callus induction, somatic embryogenesis and plant regeneration in selected Turkish crocus species

Verma, Sandeep Kumar, Das, Ashok Kumar, Cingoz, Gunce Sahin, Uslu, Emel, Gurel, Ekrem
Biotechnology reports 2016 v.10 pp. 66-74
Crocus, abscisic acid, callus, corms, culture media, explants, in vitro regeneration, leaves, plantlets, shoots, somatic embryogenesis, somatic embryos, sucrose
Callus induction, somatic embryogenesis and plant regeneration were initiated in selected five species of Turkish crocus using three diffrent explants (leaf, stem and corm) cultured on four different media (MS, GB5, LS and CHE). The highest frequencies of callus induction (100%) and shoot regeneration (70%, with 7.2 shoots/callus) were found in the crocus species Crocus oliveri ssp. Oliveri, using the MS medium containing 5% (w/v) sucrose supplemented with (4mg/L NAA+4mg/L TDZ) and (2mg/L IAA+2mg/L TDZ+2mg/L BAP). When the embryogenic calli were transferred into the four nutrient media containing (2mg/L IAA+2mg/L TDZ) and 100mg/L ABA, these further developed into cotyledonary embryos. Maximum number of somatic embryos (2.9 embryos per leaf explant, with a frequency 46.6%) was obtained in C. oliveri ssp. Oliveri. During subculture using the half strength media, cotyledonary embryos gradually developed into plantlets.