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Molecular Characterization of the Vitellogenin Receptor in Bactericera cockerelli (Sulc)1

Villanueva-Segura, O. Karina, Favela-Lara, Susana, Lopez-Monroy, Beatriz, Ponce-Garcia, Gustavo, Rodriguez-Sanchez, Iram P., Quiroz-Martinez, Humberto, Lomeli-Flores, J. Refugio, Flores, Adriana E.
The Southwestern entomologist 2016 v.41 no.3 pp. 605-612
quantitative polymerase chain reaction, sequence analysis, tomatoes, Solanum lycopersicum, Bactericera cockerelli, males, Solanum tuberosum, pest control, genetic databases, capillary electrophoresis, potatoes, transcriptome, zebra chip disease, vitellogenin, females
Potato purple top and zebra chip diseases are transmitted by potato psyllid, Bactericera cockerelli (Sulc), to tomato, Solanum lycopersicum L., and potato, Solanum tuberosum L., crops. The aim of this study was to characterize and quantify vitellogenin receptor in potato psyllid. We obtained partial characterization of the receptor by end-point PCR and sequencing using capillary electrophoresis. Relative quantification by real-time PCR was used to determine expression levels of the receptor in females and males. Similarity in vitellogenin sequence obtained in a laboratory was 89% compared with the sequence of the entire transcriptome of the species reported in GenBank. Expression analysis showed the vitellogenin receptor significantly expressed in potato psyllid females compared with males from potato plants. Further study of the vitellogenin receptor of potato psyllid is needed to determine its complete sequence and potential target to control the pest.