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Removal of 17 β-estradiol from poultry litter via solid state cultivation of lignolytic fungi

Liu, Jiayang, Luo, Qi, Huang, Qingguo
Journal of cleaner production 2016 v.139 pp. 1400-1407
Pycnoporus, bagasse, biomass, citric acid, fungi, hormones, laccase, land application, lignocellulose, poultry manure, solid state culture, sweat
Emerging contaminants like hormones can interfere ecological life health. Both liquid cultivation and direct enzymatic process have been proved effective for hormonal removal. This study aimed to assess direct removal of 17 β-estradiol (E2) from solid medium poultry litter (PL) by cultivating laccase-producing fungi toward clean treatment of PL. Before E2 removal test, laccase production was optimized by addition of citric acid and lignocellulosic biomasses into PL, which turned out to dramatically enhance laccase production to varying degrees. The highest laccase activity of approximately 16 U/g medium was produced by Pycnoporus sp. SYBC-L3 on PL mixed with sweat sorghum bagasse at a 2 to 3 (g/g) ratio. An extra dose of E2 spiked in the PL/biomass mixture (final concentration of 10 μg/g) was used to test its effect on laccase production and subsequent E2 removal. Over 80% E2 was removed from the above cultivation system, and E2 removal appeared to be positively correlated with laccase activity. Our results might present an alternative clean strategy for livestock manure treatment before land application.