Main content area

Cytological and molecular analysis of wheat – Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat

Song, Liqiang, Lu, Yuqing, Zhang, Jinpeng, Pan, Cuili, Yang, Xinming, Li, Xiuquan, Liu, Weihua, Li, Lihui
Genome 2016 v.59 no.10 pp. 840-850
Agropyron cristatum, agronomic traits, breeding, chromosome translocation, chromosomes, fluorescence in situ hybridization, genome, germplasm, leaf rust, translocation lines, wheat
Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of common wheat and confers several desirable agronomic traits to wheat, such as high grain number per spike and enhanced resistance to certain diseases. Development of wheat – A. cristatum 6P translocation lines facilitates its utilization in wheat improvement. In this study, 26 wheat – A. cristatum 6P translocation lines were characterized by in situ hybridization (ISH) and 6P-specific sequence-tagged-site (STS) markers. These translocation lines carried different 6P chromosomal segments, which covered the whole 6P chromosome. FISH results showed that 15, 5, and 6 lines were translocated onto wheat A, B, and D genomes, respectively. Compared with the previous reports, a fine physical map of 6P chromosome was constructed, consisting of 31 chromosomal bins with 255 STS markers. Twelve translocation lines containing 6PS13∼14 chromosomal bins were highly resistant to leaf rust. Two lines showed high grain number per spike, and three lines displayed both enhanced grain number per spike and thousand-grain weight. Development of wheat – A. cristatum 6P translocation lines will not only provide novel wheat germplasm for wheat breeding but also be helpful to broaden the genetic basis of common wheat.