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Cytological and molecular analysis of wheat – Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat

Author:
Song, Liqiang, Lu, Yuqing, Zhang, Jinpeng, Pan, Cuili, Yang, Xinming, Li, Xiuquan, Liu, Weihua, Li, Lihui
Source:
Genome 2016 v.59 no.10 pp. 840-850
ISSN:
1480-3321
Subject:
Agropyron cristatum, agronomic traits, breeding, chromosome translocation, chromosomes, fluorescence in situ hybridization, genome, germplasm, leaf rust, translocation lines, wheat
Abstract:
Agropyron cristatum (2n = 4x = 28, PPPP) is a wild relative of common wheat and confers several desirable agronomic traits to wheat, such as high grain number per spike and enhanced resistance to certain diseases. Development of wheat – A. cristatum 6P translocation lines facilitates its utilization in wheat improvement. In this study, 26 wheat – A. cristatum 6P translocation lines were characterized by in situ hybridization (ISH) and 6P-specific sequence-tagged-site (STS) markers. These translocation lines carried different 6P chromosomal segments, which covered the whole 6P chromosome. FISH results showed that 15, 5, and 6 lines were translocated onto wheat A, B, and D genomes, respectively. Compared with the previous reports, a fine physical map of 6P chromosome was constructed, consisting of 31 chromosomal bins with 255 STS markers. Twelve translocation lines containing 6PS13∼14 chromosomal bins were highly resistant to leaf rust. Two lines showed high grain number per spike, and three lines displayed both enhanced grain number per spike and thousand-grain weight. Development of wheat – A. cristatum 6P translocation lines will not only provide novel wheat germplasm for wheat breeding but also be helpful to broaden the genetic basis of common wheat.
Agid:
5555116