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Effects of Calcium and Magnesium Ions on Acute Copper Toxicity to Glochidia and Early Juveniles of the Chinese Pond Mussel Anodonta woodiana

Liu, Hongbo, Chen, Xiubao, Su, Yanping, Kang, Ik Joon, Qiu, Xuchun, Shimasaki, Yohei, Oshima, Yuji, Yang, Jian
Bulletin of environmental contamination and toxicology 2016 v.97 no.4 pp. 504-509
Anodonta woodiana, calcium, cations, copper, juveniles, magnesium, median effective concentration, mussels, toxicity, toxicity testing
We evaluated the effects of calcium (Ca²⁺) and magnesium (Mg²⁺) ions on copper (Cu) toxicity to glochidia and newly-transformed juvenile mussels (age 1–2 days) of the Chinese pond mussel (Anodonta woodiana). Acute Cu toxicity tests were performed with glochidia for 24 h and juveniles for 96 h with measured Ca²⁺ concentrations of 1.1, 14, 26, 51, and 99 mg L⁻¹, or measured Mg²⁺ concentrations of 2.6, 11, 21, and 39 mg L⁻¹. The Ca²⁺ and Mg²⁺ cations provided no statistically significant protection against Cu toxicity to glochidia or juveniles. The 24-h EC₅₀ value for glochidia was 82 μg L⁻¹ Cu, and contrastly, 96-h EC₅₀ value for newly-transformed juvenile mussels was as low as 12 μg L⁻¹ Cu, implying that the juveniles of A. woodiana are more vulnerable to Cu contamination at concentrations close to currently-accepted levels.