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Electrochemiluminescence (ECL) immunosensor for detection of Francisella tularensis on screen-printed gold electrode array
- Spehar-Délèze, Anna-Maria, Julich, Sandra, Gransee, Rainer, Tomaso, Herbert, Dulay, Samuel B., O’Sullivan, Ciara K.
- Analytical and bioanalytical chemistry 2016 v.408 no.25 pp. 7147-7153
- Francisella tularensis, antibodies, bacteria, detection limit, electrochemiluminescence, electrodes, electronics, gold, immunoassays, immunosensors, lipopolysaccharides, nanoparticles, pathogens, rapid methods
- An electrochemiluminescence (ECL) immunosensor for the rapid detection of the Francisella tularensis pathogen using whole antibodies or antibody fragments as capture biomolecule is described. A sandwich immunoassay was used with either lipopolysaccharide (LPS) or the whole inactivated bacterial cell (LVS) as a target, while Ru(bpy)₃ ²⁺-encapsulated silicate nanoparticles were linked to the secondary antibody and used as ECL labels. The assay was performed in a fluidic chip housed in a custom-built black box incorporating electronics, optics and fluidics. The obtained limit of detection for LPS was 0.4 ng/mL, while for the LVS it was 70 and 45 bacteria/mL when the capturing molecule was the whole antibody and the antibody F(ab) fragment, respectively.