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Protective effect of Launaea procumbens (L.) on lungs against CCl4-induced pulmonary damages in rat
- Khan, Rahmat Ali
- BMC complementary and alternative medicine 2012 v.12 no.1 pp. 133
- DNA, DNA damage, DNA fragmentation, Launaea, NAD(P)H dehydrogenase (quinone), alternative medicine, antioxidant activity, antioxidants, body weight, carbon tetrachloride, catalase, dimethyl sulfoxide, enzyme activity, glutathione, glutathione peroxidase, glutathione transferase, glutathione-disulfide reductase, histopathology, humans, lipid peroxidation, lungs, males, methanol, olive oil, oxidative stress, peroxidase, protective effect, rats, rutin, superoxide dismutase, thiobarbituric acid-reactive substances
- BACKGROUND: Launaea procumbens (L.) is traditionally used in the treatment of various human ailments including pulmonary damages. The present study was arranged to evaluate the role of Launaea procumbens methanol extract (LME) against carbon tetrachloride (CCl₄) induced oxidative pulmonary damages in rat. METHODS: 36 Sprague–Dawley male rats (170-180 g) were randomly divided into 06 groups. After a week of acclamization, group I was remained untreated while group II was given olive oil intraperitoneally (i.p.) and dimethyl sulfoxide (DMSO) orally, groups III, IV, V and VI were administered CCl₄, 3 ml/kg body weight (30% in olive oil i.p.). Groups IV, V were treated with 100 mg/kg, 200 mg/kg of LME whereas group VI was administered with 50 mg/kg body weight of rutin (RT) after 48 h of CCl₄ treatment for four weeks. Antioxidant profile in lungs were evaluated by estimating the activities of antioxidant enzymes; catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione reductase (GSR), glutathione peroxidase (GSH-Px), quinone reductase (QR) and reduced glutathione (GSH). CCl₄-induced lipid peroxidation was determined by measuring the level of thiobarbituric acid reactive substances (TBARS) with conjugation of deoxyribonucleic acid (DNA) damages, argyrophilic nucleolar organizer regions (AgNORs) counts and histopathology. RESULTS: Administration of CCl₄ for 6 weeks significantly (p < 0.01) reduced the activities of antioxidant enzymes and GSH concentration while increased TBARS contents and DNA damages in lung samples. Co-treatment of LME and rutin restored the activities of antioxidant enzymes and GSH contents. Changes in TBARS concentration and DNA fragmentation were significantly (p < 0.01) decreased with the treatment of LME and rutin in lung. Changes induced with CCl₄ in histopathology of lungs were significantly reduced with co-treatment of LME and rutin. CONCLUSION: Results of present study revealed that LME could protect the lung tissues against CCl₄-induced oxidative stress possibly by improving the antioxidant defence system.