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Physical mapping of NBS-coding resistance genes to the Me-gene cluster on chromosome P9 reveals markers tightly linked to the N gene for root-knot nematode resistance in pepper

Author:
Celik, Ibrahim, Sogut, Mehmet Ali, Ozkaynak, Ercan, Doganlar, Sami, Frary, Anne
Source:
Molecular breeding 2016 v.36 no.10 pp. 137
ISSN:
1380-3743
Subject:
Capsicum annuum, chromosomes, genes, marker-assisted selection, microsatellite repeats, pepper, pest resistance, root-knot nematodes
Abstract:
Natural root-knot nematode resistance genes are unique resources to control this major pest in pepper (Capsicum annuum). Although four genes (Me1, Me3, Me7 and N) conferring broad-spectrum resistance were mapped to a cluster in a 28-cm interval on chromosome P9, limited markers targeting this region were available. In the present study, the Me-gene cluster was structurally annotated for resistance genes to develop markers targeting the N gene. As a result, the Me-gene cluster (4.07 Mb in size) was found to contain three resistance gene hotspots. In addition, a SSR maker tightly linked to the N gene (0.8 cM away) was developed for marker-assisted selection in pepper.
Agid:
5556363