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Increased expression of native cytosolic Cu/Zn superoxide dismutase and ascorbate peroxidase improves tolerance to oxidative and chilling stresses in cassava (Manihot esculenta Crantz)

Author:
Xu, Jia, Yang, Jun, Duan, Xiaoguang, Jiang, Yueming, Zhang, Peng
Source:
BMC plant biology 2014 v.14 no.1 pp. 208
ISSN:
1471-2229
Subject:
Manihot esculenta, ascorbate peroxidase, cassava, catalase, chlorophyll, cold stress, cold tolerance, defense mechanisms, enzymatic reactions, enzyme activity, gene expression regulation, glutathione-disulfide reductase, homeostasis, hydrogen peroxide, leaves, lipid peroxidation, malondialdehyde, monodehydroascorbate reductase (NADH), oxidative stress, paraquat, reverse transcriptase polymerase chain reaction, superoxide dismutase, temperature, transgenic plants
Abstract:
BACKGROUND: Cassava (Manihot esculenta Crantz) is a tropical root crop, and is therefore, extremely sensitive to low temperature; its antioxidative response is pivotal for its survival under stress. Timely turnover of reactive oxygen species (ROS) in plant cells generated by chilling-induced oxidative damages, and scavenging can be achieved by non-enzymatic and enzymatic reactions in order to maintain ROS homeostasis. RESULTS: Transgenic cassava plants that co-express cytosolic superoxide dismutase (SOD), MeCu/ZnSOD, and ascorbate peroxidase (APX), MeAPX2, were produced and tested for tolerance against oxidative and chilling stresses. The up-regulation of MeCu/ZnSOD and MeAPX2 expression was confirmed by the quantitative reverse transcriptase-polymerase chain reaction, and enzymatic activity analyses in the leaves of transgenic cassava plant lines with a single-transgene integration site. Upon exposure to ROS-generating agents, 100 μM ROS-generating reagent methyl viologen and 0.5 M H₂O₂, higher levels of enzymatic activities of SOD and APX were detected in transgenic plants than the wild type. Consequently, the oxidative stress parameters, such as lipid peroxidation, chlorophyll degradation and H₂O₂ synthesis, were lower in the transgenic lines than the wild type. Tolerance to chilling stress at 4°C for 2 d was greater in transgenic cassava, as observed by the higher levels of SOD, catalase, and ascorbate-glutathione cycle enzymes (e.g., APX, monodehydroascorbate reductase, dehydroascorbate reducatase and glutathione reductase) and lower levels of malondialdehyde content. CONCLUSIONS: These results suggest that the expression of native cytosolic SOD and APX simultaneously activated the antioxidative defense mechanisms via cyclic ROS scavenging, thereby improving its tolerance to cold stress.
Agid:
5557689