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Arcobacter acticola sp. nov., isolated from seawater on the East Sea in South Korea

Park, Sooyeon, Jung, Yong-Taek, Kim, Sona, Yoon, Jung-Hoon
The journal of microbiology 2016 v.54 no.10 pp. 655-659
Arcobacter, DNA, bacteria, fatty acids, menaquinones, nucleotide sequences, pH, phenotype, phosphatidylethanolamines, phylogeny, ribosomal RNA, seawater, sequence homology, sodium chloride, South Korea
A Gram-stain-negative, facultative aerobic, non-flagellated, and rod-shaped bacterium, designated AR-13ᵀ, was isolated from a seawater on the East Sea in South Korea, and subjected to a polyphasic taxonomic study. Strain AR-13ᵀ grew optimally at 30°C, at pH 7.0–8.0 and in the presence of 0–0.5% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain AR-13ᵀ fell within the clade comprising the type strains of Arcobacter species, clustering coherently with the type strain of Arcobacter venerupis. Strain AR-13ᵀ exhibited 16S rRNA gene sequence similarity values of 98.1% to the type strain of A. venerupis and of 93.2–96.9% to the type strains of the other Arcobacter species. Strain AR-13ᵀ contained MK-6 as the only menaquinone and summed feature 3 (C₁₆:₁ ω7c and/or C₁₆:₁ ω6c), C₁₆:₀, C₁₈:₁ ω7c, and summed feature 2 (iso-C₁₆:₁ I and/or C₁₄:₀ 3-OH) as the major fatty acids. The major polar lipids detected in strain AR-13ᵀ were phosphatidylethanolamine, phosphatidylglycerol, and one unidentified aminophospholipid. The DNA G+C content was 28.3 mol% and its mean DNA-DNA relatedness value with the type strain of A. venerupis was 21%. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain AR-13ᵀ is separated from recognized Arcobacter species. On the basis of the data presented, strain AR-13ᵀ is considered to represent a novel species of the genus Arcobacter, for which the name Arcobacter acticola sp. nov. is proposed. The type strain is AR-13ᵀ (=KCTC 52212ᵀ =NBRC 112272ᵀ).