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Determination of the infectious titer and virulence of an original US porcine epidemic diarrhea virus PC22A strain
- Liu, Xinsheng, Lin, Chun-Ming, Annamalai, Thavamathi, Gao, Xiang, Lu, Zhongyan, Esseili, Malak A, Jung, Kwonil, El-Tholoth, Mohamed, Saif, Linda J, Wang, Qiuhong
- Veterinary research 2015 v.46 no.1 pp. 109
- Porcine epidemic diarrhea virus, cell culture, diarrhea, piglets, sows, suckling, vaccines, virulence, viruses, United States
- The infectious dose of a virus pool of original US PEDV strain PC22A was determined in 4-day-old, cesarean-derived, colostrum-deprived (CDCD) piglets. The median pig diarrhea dose (PDD₅₀) of the virus pool was determined as 7.35 log₁₀ PDD₅₀/mL, similar to the cell culture infectious titer, 7.75 log₁₀ plaque-forming units (PFU)/mL. 100 PDD₅₀ caused watery diarrhea in all conventional suckling piglets (n = 12) derived from a PEDV-naive sow, whereas 1000 and 10 000 PDD₅₀ did not cause diarrhea in piglets derived from two PEDV-field exposed-recovered sows. This information is important for future PEDV challenge studies and validation of PEDV vaccines.