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Reusable nanobiocatalysts for the efficient extraction of pigments from orange peel
- Kumar, Sanjay, Sharma, Pramila, Ratrey, Poonam, Datta, Bhaskar
- Journal of food science and technology 2016 v.53 no.7 pp. 3013-3019
- Citrus reticulata, endo-1,4-beta-glucanase, glutaraldehyde, hydrolysis, iron oxides, mandarins, nanoparticles, orange peels, pH, pigments, polygalacturonase, solvents, temperature
- Nagpur mandarin orange (Citrus reticulata) peels were subjected to treatment with nanobiocatalysts in the form of cellulase and pectinase immobilized magnetic nanoparticles (MNPs). MNPs (Fe₃O₄) with average diameter in range of 40–90 nm were immobilized with cellulase and pectinase through APTES and glutaraldehyde. Treatment followed by extraction into organic solvents resulted in 8-9 fold increase in extraction of carotenoidic pigments compared to use of free enzymes. Optimum pH and temperature for the process were determined to be 5.0 and 50 °C, respectively. The nanobiocatalysts could be reused across three cycles with only 15 % drop in yield per cycle. Dinitrosalicylic acid assays showed that superior peel hydrolysis also led to greatest extent of pigment extraction.