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Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody

Matsubayashi, Makoto, Minoura, Chisa, Kimura, Shintaro, Tani, Hiroyuki, Furuya, Masaru, Lillehoj, Hyun S., Matsuda, Haruo, Takenaka, Shigeo, Hatta, Takeshi, Tsuji, Naotoshi, Sasai, Kazumi
Parasitology research 2016 v.115 no.11 pp. 4123-4128
Cryptosporidium parvum, Eimeria acervulina, Neospora, Toxoplasma, Western blotting, antigens, chickens, cytoskeleton, monoclonal antibodies, pathogens
In the poultry industry, Eimeria spp. is one of the important pathogens which cause significant economic losses. We have previously generated a chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for an antigen located in the apical cytoskeleton of Eimeria acervulina and with cross-reactive among Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp. Furthermore, the protein of Cryptosporidium parvum recognized by the 6D-12-G10 has been identified as elongation factor-1α (EF-1α). In the present study, to identify the target molecule of E. acervulina by the mAb, we performed two-dimensional Western blotting analysis. Finally, we found two positive molecules which are identified as EF-1α and a related protein. Our previous finding using C. parvum and the results in this study suggest that EF-1α could be associated with the invasion facilitated by the cytoskeleton at the apical region of zoites.