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Identification of Eimeria acervulina conoid antigen using chicken monoclonal antibody
- Matsubayashi, Makoto, Minoura, Chisa, Kimura, Shintaro, Tani, Hiroyuki, Furuya, Masaru, Lillehoj, Hyun S., Matsuda, Haruo, Takenaka, Shigeo, Hatta, Takeshi, Tsuji, Naotoshi, Sasai, Kazumi
- Parasitology research 2016 v.115 no.11 pp. 4123-4128
- Cryptosporidium parvum, Eimeria acervulina, Neospora, Toxoplasma, Western blotting, antigens, chickens, cytoskeleton, monoclonal antibodies, pathogens
- In the poultry industry, Eimeria spp. is one of the important pathogens which cause significant economic losses. We have previously generated a chicken monoclonal antibody (mAb), 6D-12-G10, with specificity for an antigen located in the apical cytoskeleton of Eimeria acervulina and with cross-reactive among Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp. Furthermore, the protein of Cryptosporidium parvum recognized by the 6D-12-G10 has been identified as elongation factor-1α (EF-1α). In the present study, to identify the target molecule of E. acervulina by the mAb, we performed two-dimensional Western blotting analysis. Finally, we found two positive molecules which are identified as EF-1α and a related protein. Our previous finding using C. parvum and the results in this study suggest that EF-1α could be associated with the invasion facilitated by the cytoskeleton at the apical region of zoites.