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Determining exon connectivity in complex mRNAs by nanopore sequencing

Author:
Bolisetty, Mohan T., Rajadinakaran, Gopinath, Graveley, Brenton R.
Source:
Genome biology 2015 v.16 no.1 pp. 204
ISSN:
1474-760X
Subject:
Drosophila, exons, genes, high-throughput nucleotide sequencing, messenger RNA, nanopores, transcriptome
Abstract:
Short-read high-throughput RNA sequencing, though powerful, is limited in its ability to directly measure exon connectivity in mRNAs that contain multiple alternative exons located farther apart than the maximum read length. Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 ‘full-length’ isoforms expressed from four Drosophila genes, Dscam1, MRP, Mhc, and Rdl. These results demonstrate that nanopore sequencing can be used to deconvolute individual isoforms and that it has the potential to be a powerful method for comprehensive transcriptome characterization.
Agid:
5559727