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Nanoformulation of curcumin protects HUVEC endothelial cells against ionizing radiation and suppresses their adhesion to monocytes: potential in prevention of radiation-induced atherosclerosis
- Soltani, Behrooz, Bodaghabadi, Narges, Mahpour, Gita, Ghaemi, Nasser, Sadeghizadeh, Majid
- Biotechnology letters 2016 v.38 no.12 pp. 2081-2088
- adhesion, atherosclerosis, curcumin, gamma radiation, glutathione, human umbilical vein endothelial cells, humans, inflammation, interleukin-6, interleukin-8, irradiation, messenger RNA, mitogen-activated protein kinase, monocytes, thiobarbituric acid-reactive substances, transcription factor NF-kappa B, viability
- OBJECTIVES: To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS: Co⁶⁰ gamma rays reduced viability, raised the expression of adhesion molecules, ICAM-1, VCAM-1 and E-selectin (mRNA and protein), augmented the adhesion of THP-1 cells to HUVECs, activated NF-κB binding, increased the release of pro-inflammatory cytokines (IL-6, IL-8 and MCP-1) and induced oxidative damage (reduced glutathione declined, while 8-OHdG and TBARS increased). 5 µM DNC significantly inhibited these radiation-induced changes, activated the Nrf-2 pathway, and effectively suppressed THP-1 adhesion to HUVECs, implicating p38 MAPK signaling. CONCLUSION: DNC treatment is a potential preventive method against inflammation and vascular damage from ionizing radiation.