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Generation of ΔF508-CFTR T84 cell lines by CRISPR/Cas9-mediated genome editing

Author:
Chung, Woo Young, Song, Myungjae, Park, Jinhong, Namkung, Wan, Lee, Jinu, Kim, Hyongbum, Lee, Min Goo, Kim, Joo Young
Source:
Biotechnology letters 2016 v.38 no.12 pp. 2023-2034
ISSN:
0141-5492
Subject:
screening, protein degradation, protein folding, physiological transport, temperature, RNA, exons
Abstract:
Objectives: To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. Results: CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels that abolished CFTR protein expressions. Next, stable ΔF508-CFTR expression was achieved by genome integration of ΔF508-CFTR via the lentivirus infection system. Finally, we showed functional rescue of ΔF508-CFTR not only by growing the cells at a low temperature, but also incubating with VX-809, a ΔF508-CFTR corrector, in the established T84 cells expressing ΔF508-CFTR. Conclusions: This cell system provides an appropriate screening platform for rescue of ΔF508-CFTR, especially related to protein folding, escaped from endoplasmic-reticulum-associated protein degradation, and membrane transport.
Agid:
5561919