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Function-spacer-lipid constructs of Lewis and chimeric Lewis/ABH glycans. Synthesis and use in serological studies

Author:
Ryzhov, Ivan M., Korchagina, Elena Yu., Tuzikov, Alexander B., Popova, Inna S., Tyrtysh, Tatiana V., Pazynina, Galina V., Henry, Stephen M., Bovin, Nicolai V.
Source:
Carbohydrate research 2016 v.435 pp. 83-96
ISSN:
0008-6215
Subject:
blood groups, chimerism, erythrocytes, glycosides, immunochemistry, oligosaccharides, polysaccharides, serological surveys
Abstract:
Seven lipophilic constructs containing Lewis (Lea, Leb, Ley) or chimeric Lewis/ABH (ALeb, BLeb, ALey, BLey) glycans were obtained starting from corresponding oligosaccharides in form of 3-aminopropyl glycosides. ALeb and BLeb pentasaccharides were synthesized via [3 + 1] blockwise approach. The constructs (neoglycolipids, or FSLs) were inserted in erythrocyte membrane, and obtained “kodecytes” were used to map the immunochemical specificity of historical and contemporary monoclonal and polyclonal blood group system Lewis reagents.
Agid:
5563970