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Glycosynthase mediated synthesis of psychosine
- Goddard-Borger, Ethan D., Tysoe, Christina, Withers, Stephen G.
- Carbohydrate research 2016 v.435 pp. 97-99
- Rhodococcus equi, blood sampling, early diagnosis, enzyme substrates, fluorides, isotope labeling, mass spectrometry, mutants, neonates, sphingosine
- Globoid cell leukodystrophy (GCL), or Krabbe disease, is a lysosomal storage disorder characterized by a deficiency in galactosylceramidase (GALC), which hydrolyses galactosylceramide and galactosylsphingosine (psychosine). Early detection of GCL in newborns is essential for timely therapeutic intervention and could be achieved by testing infant blood samples with isotopically labeled lysosmal enzyme substrates and mass spectrometry. While isotopically labeled psychosine would be a useful tool for the early diagnosis of GCL, its synthesis is lengthy and expensive. To obviate this problem we developed a one-step chemoenzymatic synthesis of psychosine using a glycosynthase mutant of the Rhodococcus equi endogalactosylceramidase (EGALC), α-D-galactopyranosyl fluoride and sphingosine.