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A preconcentration method for indirect determination of acrylamide from chips, crackers and cereal-based baby foods using flame atomic absorption spectrometry

Altunay, Nail, Gürkan, Ramazan, Orhan, Ulaş
Talanta 2016 v.161 pp. 143-150
Maillard reaction, acrylamides, ammonia, ammonium chloride, atomic absorption spectrometry, buffers, children, cost effectiveness, crackers, detection limit, fluorescein, heat treatment, human health, infant foods, ions, nickel, pH, processed foods, statistical analysis, toxicity
Acrylamide is a toxic species for human health, and is a Maillard reaction product which forms spontaneously in heat treatment process of foods. Therefore, a simple, fast and cost-effective method was developed for the indirect determination of acrylamide in processed foods particularly consumed by children. The method is based on ion–pairing of acrylamide with fluorescein (F2−) in presence of Ni(II) ions at pH 9.0, and then extraction of the formed ternary complex into micellar phase of poly(ethyleneglycol-mono-p-nonylphenylether) (PONPE 7.5) before analysis by flame atomic absorption spectrometry (FAAS). The ultrasonic-assisted cloud point extraction (UA-CPE) has been used for the preconcentration of acrylamide in the samples prior to its FAAS detection. The matrix matched calibration curve is linear in range of 0.3–150µgkg−1 under optimal reagent conditions (1.75mL of 0.1molL−1 ammonia buffer at pH 9.0, 2.2mgL−1 Ni(II), 4.0×10−4molL−1 F2−, 0.4% (w/v) NH4Cl and 0.7% (v/v) PONPE 7.5) with sensitivity enhancement of 160-fold. The proposed method has been validated by assessment of the following parameters; the limits of detection (LOD) and quantification (LOQ) (0.08µgkg−1 and 0.28µgkg−1, respectively) with a relative standard deviation (RSD%) lower than 6.3%, and extractive recovery higher than 95% for acrylamide spiked at levels of 5 and 25µgkg−1. The method was successfully applied to the indirect determination of acrylamide in the processed foods and two CRMs with satisfactory results.