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Complete genome sequence of plant growth-promoting bacterium Leifsonia xyli SE134, a possible gibberellin and auxin producer

Author:
Kang, Sang-Mo, Asaf, Sajjad, Kim, Sang-Jun, Yun, Byung-Wook, Lee, In-Jung
Source:
Journal of biotechnology 2016 v.239 pp. 34-38
ISSN:
0168-1656
Subject:
Leifsonia xyli, biosynthesis, carbohydrate metabolism, chromosomes, energy, genes, genomics, gibberellins, growth promotion, indole acetic acid, nucleotide sequences, nutrient deficiencies, oxidative stress, plant growth, plant growth-promoting rhizobacteria, ribosomal RNA, root exudates, secretion, siderophores, soil, trehalose, South Korea
Abstract:
Leifsonia xyli SE134 is a potential plant growth-promoting bacterium isolated from a soil in Daegu, Republic of Korea, which produces large amounts of gibberellin (GA) and indole acetic acid (IAA). In this study, we sequenced the complete genome of L. xyli SE134 by the Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. The genome of L. xyli SE134 contains a single chromosome that is 3,596,761bp in length, with 70.2% G+C content. The genome contains 3466 protein-coding genes (CDSs) and 51 rRNA- and 46 tRNA-coding genes. By genomic analysis, we identified genes that are potentially involved in plant growth promotion such as genes participating in indole-3-acetic acid (IAA) biosynthesis, siderophore, and trehalose production. L. xyli SE134 also harbours genes for central carbohydrate metabolism, indicating that it can utilise the root exudates with other organic materials as an energy source. Furthermore, the SE134 genome is equipped with various kinds of genes for adaptation to plant surfaces, e.g. defence against desiccation, nutrient deficiencies, and oxidative stress, and a large proportion of genes related to secretion mechanisms and signalling. The genetic information provided here may help to expand this bacterium’s biotechnological potential and to further improve its plant growth-promoting characteristics.
Agid:
5570149