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Phenolics and abscisic acid identified in acacia honey comparing different SPE cartridges coupled with HPLC-PDA

Sun, Chunli, Tan, Hongbo, Zhang, Yuhao, Zhang, Hongcheng
Journal of food composition and analysis 2016 v.53 pp. 91-101
Acacia, abscisic acid, adulterated products, anion exchange, apiaries, flavonoids, food matrix, high performance liquid chromatography, honey, ingredients, phenolic compounds, quality control, solid phase extraction, sugars, China
The major components of honey are sugar and water, but some phenolic compounds may be responsible for honey quality. We investigated the effects of four reversed-phase (RP) and four reversed-phase and anion-exchange (RP-AE) solid-phase extraction (SPE) cartridges as a pre-concentration technique for honey phenolics. Eleven acacia honey samples were collected from different apiaries of China and their levels of phenolics and abscisic acid were analyzed using high-performance liquid chromatography (HPLC). Our results reveal that RP-AE SPE cartridges are superior to RP SPE cartridges for the pre-concentration of honey phenolics. The improved pre-concentration effect of RP-AE SPE cartridges may be a novel finding of our research. The Strata-X-A cartridge may be used in the concentration of low content phenolics of complex food matrices. We identified cis-trans-abscisic acid and 19 phenolics occurring in acacia honey samples. Seven phenolic compounds and abscisic acid were identified in almost all samples, representing 50% of total phenolics. Abscisic acid possesses the highest average content of 146.0μg/100g, and pinobanksin also presented the highest average of 53.1μg/100g among all flavonoids. Moreover, we infer that abscisic acid could be the solid ingredient for adulteration identification and quality control of acacia honey.