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The kinetic study of isotretinoin release from nanoemulsion A Physicochemical and engineering aspects

Miastkowska, Małgorzata, Sikora, Elżbieta, Ogonowski, Jan, Zielina, Michał, Łudzik, Agnieszka
Colloids and surfaces 2016 v.510 pp. 63-68
Propionibacterium acnes, acne, adverse effects, ambient temperature, blood serum, cellulose, coconut oil, droplet size, drugs, keratinization, kinetics, light scattering, nanoemulsions, polysorbates, sebum, skin irritation, spectroscopy
Acne is an inflammatory dermatosis caused by excessive production of sebum, abnormal follicular keratinization and Propionibacterium acnes. Isotretinoin is a retinoid, one of known topical delivered drugs, used in acne treatment because it normalizes the keratinization process. For greatest efficacy in dermatosis treatment, vehicles which enhance delivery of the active to the skin without reaching high serum concentrations and not causing skin irritation are still requested.The aim of this work was to develop a nanoemulsion formulation as a carrier for topical delivery of isotretinoin and determine the release kinetic of the active from the nanoemulsion.O/W isotretinoin-loaded nanoemulsions (0.05% wt.) based on coconut oil and stabilized by a Polysorbate 80 was obtained and studied as isotretinon delivery systems. The nanoemulsions were prepared by stepwise addition of water to the mixture of polysorbate/oil/the retinoid, at room temperature. The mean droplet size of the nanoemulsions measured by Dynamic Light Scattering (DLS) was around 21nm. The drug release study of the active was carried out using the Spectra/Por Standard Regenerated Cellulose (RC) membrane, at the temperature T=32°C. The concentration of isotretinoin in the receptor solution was analyzed by UV–vis spectroscopy method. The release was found to follow the zero order kinetic model. The obtained nanoemulsion effect on isotretinoin release therefore can be used as a vehicle for controlled drug release which resulting in prolonged dermatological action and reduce side effects.