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Light sheet microscopes: Novel imaging toolbox for visualizing life’s processes

Author:
Heddleston, John M., Chew, Teng-Leong
Source:
The international journal of biochemistry & cell biology 2016 v.80 pp. 119-123
ISSN:
1357-2725
Subject:
fluorescence, image analysis, light microscopy, microscopes
Abstract:
Capturing dynamic processes in live samples is a nontrivial task in biological imaging. Although fluorescence provides high specificity and contrast compared to other light microscopy techniques, the photophysical principles of this method can have a harmful effect on the sample. Current advances in light sheet microscopy have created a novel imaging toolbox that allows for rapid acquisition of high-resolution fluorescent images with minimal perturbation of the processes of interest. Each unique design has its own advantages and limitations. In this review, we describe several cutting edge light sheet microscopes and their optimal applications.
Agid:
5575011