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Drug Affinity Responsive Target Stability (DARTS) Identifies Laurifolioside as a New Clathrin Heavy Chain Modulator

Author:
Dal Piaz, Fabrizio, Vera Saltos, Mariela Beatriz, Franceschelli, Silvia, Forte, Giovanni, Marzocco, Stefania, Tuccinardi, Tiziano, Poli, Giulio, Nejad Ebrahimi, Samad, Hamburger, Matthias, De Tommasi, Nunziatina, Braca, Alessandra
Source:
Journal of natural products 2016 v.79 no.10 pp. 2681-2692
ISSN:
1520-6025
Subject:
Euphorbia, aerial parts, clathrin, diterpenoids, drugs, microscopy, molecular dynamics, molecular models, nuclear magnetic resonance spectroscopy, plasma membrane, proteomics, viruses
Abstract:
Five new diterpenes (1–5) and a megastigmane derivative (6) were isolated from the aerial parts of Euphorbia laurifolia, along with several known compounds. Their structures were elucidated by NMR, MS, and ECD and by chemical methods. A chemical proteomics drug affinity responsive target stability (DARTS) approach to investigate the lathyrane diterpene 1, laurifolioside, on its putative cellular target(s) was performed. Clathrin heavy chain 1, a protein mainly involved in selective uptake of proteins, viruses, and other macromolecules at the plasma membrane of cells, was identified as the major interaction partner of compound 1. The modulation of clathrin activity by 1 was studied through microscopy, molecular docking, and molecular dynamics studies, suggesting a new activity of lathyrane diterpenes in the modulation of trafficking pathways.
Agid:
5578761