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Permanent colonization of creek sediments, creek water and limnic water plants by four Listeria species in low population densities

Lang-Halter, Evi, Schober, Steffen, Scherer, Siegfried
Zeitschrift für Naturforschung 2016 v.71 no.9-10 pp. 335-345
Fourier transform infrared spectroscopy, Listeria, Ranunculus, biodiversity, freshwater, habitats, longitudinal studies, most probable number technique, population density, random amplified polymorphic DNA technique, sediments, streams
During a 1-year longitudinal study, water, sediment and water plants from two creeks and one pond were sampled monthly and analyzed for the presence of Listeria species. A total of 90 % of 30 sediment samples, 84 % of 31 water plant samples and 67 % of 36 water samples were tested positive. Generally, most probable number counts ranged between 1 and 40 g⁻¹, only occasionally >110 cfu g⁻¹ were detected. Species differentiation based on FT-IR spectroscopy and multiplex PCR of a total of 1220 isolates revealed L. innocua (46 %), L. seeligeri (27 %), L. monocytogenes (25 %) and L. ivanovii (2 %). Titers and species compositions were similar during all seasons. While the species distributions in sediments and associated Ranunculus fluitans plants appeared to be similar in both creeks, RAPD typing did not provide conclusive evidence that the populations of these environments were connected. It is concluded that (i) the fresh-water sediments and water plants are year-round populated by Listeria, (ii) no clear preference for growth in habitats as different as sediments and water plants was found and (iii) the RAPD-based intraspecific biodiversity is high compared to the low population density.