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Temperature-dependent regulation of gene expression in Japanese flounder Paralichthys olivaceus kidney after Edwardsiella tarda formalin-killed cells
- Kaneshige, Norie, Jirapongpairoj, Walissara, Hirono, Ikuo, Kondo, Hidehiro
- Fish & shellfish immunology 2016 v.59 pp. 298-304
- Edwardsiella tarda, Paralichthys olivaceus, flounder, gene expression, gene expression regulation, genes, immune system, kidneys, messenger RNA, pathogens, quantitative polymerase chain reaction, rearing, signal transduction, temperature
- Temperature affects the activities of the immune system and the susceptibility of fish to pathogens. To investigate the modulation of temperature on immune related gene expression in formalin-killed cells (FKC) of Edwardsiella tarda-injected Japanese flounder Paralichthys olivaceus, fish reared at 15 or 22 °C were injected with FKC of E. tarda. The up-regulation of immune related genes was detected in FKC-injected fish at both temperatures by qPCR. The mRNA expression of IFNγ was highly up-regulated at 6 h post injection (hpi) in FKC-injected fish at 15 °C, whereas at 22 °C, strong up-regulation of the gene was detected at 3 hpi The mRNA expression level of IRF1 was detected from 3 hpi to day 14 post injection in fish reared at 15 °C, but the gene was up-regulated from 3 to 6 hpi in fish reared at 22 °C. Comprehensive gene expression profiling showed that immune related genes are differentially expressed between 15 and 22 °C. Genes involved in the IFNγ signaling pathway were up-regulated at 22 °C but not at 15 °C. These results demonstrate that gene(s) involved in IFNγ signaling pathway in Japanese flounder stimulated with FKC of E. tarda are regulated by temperature.