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Biodegradation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT) by using Serratia marcescens NCIM 2919 Part B Pesticides, food contaminants, and agricultural wastes

Author:
, Neerja, Grewal, Jasneet, Bhattacharya, Amrik, Kumar, Sumit, Singh, Dileep K., Khare, Sunil K.
Source:
Journal of environmental science and health 2016 v.51 no.12 pp. 809-816
ISSN:
1532-4109
Subject:
DDD (pesticide), DDE (pesticide), DDT (pesticide), Fourier transform infrared spectroscopy, Serratia marcescens, bacteria, biodegradation, ethane, metabolites, mineralization, polluted soils, solvents, toxicity
Abstract:
A solvent tolerant bacterium Serratia marcescens NCIM 2919 has been evaluated for degradation of DDT (1,1,1-trichloro-2,2- bis (4-chlorophenyl) ethane). The bacterium was able to degrade up to 42% of initial 50 mg L ⁻¹ of DDT within 10 days of incubation. The highlight of the work was the elucidation of DDT degradation pathway in S. marcescens. A total of four intermediates metabolites viz. 2,2- bis (chlorophenyl)-1,1-dichloroethane (DDD), 2,2- bis (chlorophenyl)-1,1-dichloroethylene (DDE), 2,2- bis (chlorophenyl)-1-chloroethylene (DDMU), and 4-chlorobenzoic acid (4-CBA) were identified by GC-Mass and FTIR. 4-CBA was found to be the stable product of DDT degradation. Metabolites preceding 4-CBA were not toxic to strain as reveled through luxuriant growth in presence of varying concentrations of exogenous DDD and DDE. However, 4-CBA was observed to inhibit the growth of bacterium. The DDT degrading efficiency of S. marcescens NCIM 2919 hence could be used in combination with 4-CBA utilizing strains either as binary culture or consortia for mineralization of DDT. Application of S. marcescens NCIM 2919 to DDT contaminated soil, showed 74.7% reduction of initial 12.0 mg kg ⁻¹ of DDT after 18-days of treatment.
Agid:
5588722