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Cytotoxic Lanostanoids from Poria cocos

Author:
Lai, Kuei-Hung, Lu, Mei-Chin, Du, Ying-Chi, El-Shazly, Mohamed, Wu, Tung-Ying, Hsu, Yu-Ming, Henz, Astrid, Yang, Juan-Cheng, Backlund, Anders, Chang, Fang-Rong, Wu, Yang-Chang
Source:
Journal of natural products 2016 v.79 no.11 pp. 2805-2813
ISSN:
1520-6025
Subject:
Wolfiporia cocos, cytotoxicity, global positioning systems, inhibitory concentration 50, molting, neoplasms, sclerotia, spectral analysis
Abstract:
Six new and 16 known lanostanoids were isolated from the sclerotia of Poria cocos. The structures of the new isolates were elucidated to be 16α-hydroxy-3-oxo-24-methyllanosta-5,7,9(11),24(31)-tetraen-21-oic acid (1), 3β,16α,29-trihydroxy-24-methyllanosta-7,9(11),24(31)-trien-21-oic acid (2), 3β,16α,30-trihydroxy-24-methyllanosta-7,9(11),24(31)-trien-21-oic acid (3), 3β-acetoxy-16α,24β-dihydroxylanosta-7,9(11),25-trien-21-oic acid (4), 3β,16α-dihydroxy-7-oxo-24-methyllanosta-8,24(31)-dien-21-oic acid (5), and 3α,16α-dihydroxy-7-oxo-24-methyllanosta-8,24(31)-dien-21-oic acid (6), based on extensive spectroscopic analyses. The absolute configuration of 4 was determined using Mosher’s method. The antiproliferative activity of the isolated compounds (except 3 and 4) was evaluated against four leukemic cell lines (Molt 4, CCRF-CEM, HL 60, and K562). Dehydropachymic acid (9), dehydroeburicoic acid (12), pachymic acid (14), and lanosta-7,9(11),24-trien-21-oic acid (20) exhibited an antiproliferative effect on the CCRF-CEM cancer cell line with IC₅₀ values of 2.7, 6.3, 4.9, and 13.1 μM, respectively. Both dehydropachymic acid (9) and dehydroeburicoic acid (12) showed antiproliferative effects against Molt 4 (IC₅₀ 13.8 and 14.3 μM) and HL 60 (IC₅₀ 7.3 and 6.0 μM) leukemic cell lines. Primary computational analysis using a chemical global positioning system for natural products (ChemGPS-NP) on the active lanostanoids from P. cocos suggested that targets other than topoisomerases may be involved in the antiproliferative activity.
Agid:
5590300