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Hyaluronate–Gold Nanorod/DR5 Antibody Complex for Noninvasive Theranosis of Skin Cancer

Lee, Hwiwon, Lee, Jung Ho, Kim, Jeesu, Mun, Jong Hwan, Chung, Junho, Koo, Heebeom, Kim, Chulhong, Yun, Seok Hyun, Hahn, Sei Kwang
ACS Applied Materials & Interfaces 2016 v.8 no.47 pp. 32202-32210
antibodies, antineoplastic activity, apoptosis, death domain receptors, enzyme-linked immunosorbent assay, image analysis, light scattering, luciferase, mice, models, nanorods, neoplasm cells, nuclear magnetic resonance spectroscopy, patient compliance, skin neoplasms, transmission electron microscopy, ultraviolet-visible spectroscopy, zeta potential
Noninvasive transdermal delivery is a promising method with distinct advantages including patient compliance over other delivery routes. Here, hyaluronate–gold nanorod/death receptor 5 antibody (HA-AuNR/DR5 Ab) complex was developed for transdermal theranosis of skin cancer. The successful formation of the complex was corroborated by ¹H nuclear magnetic resonance, UV–vis spectroscopy, dynamic light scattering, zeta potential, and transmission electron microscopy. In vitro biological activity of the complex was verified by ELISA and MTT assay using HCT116 cancer cells. In addition, in vivo photoacoustic imaging and two-photon microscopy clearly visualized the transdermal delivery of HA-AuNR/DR5 Ab complex through the inevitable barrier of stratum corneum in the skin. Furthermore, in vivo antitumor effect on skin cancer model mice was confirmed from statistically significant decrease of tumor-reflecting luciferase expression levels and apoptotic signals in terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Taken together, we could confirm the feasibility of HA-AuNR/DR5 Ab complex as a novel theranostic platform for noninvasive transdermal treatment of skin cancers.