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An Improved in Vitro Propagation Protocol of Nyctanthus arbor-tristis
- Shekhawat, Mahipal S., Manokari, M., Ravindran, C. P.
- Journal of herbs, spices & medicinal plants 2016 v.22 no.4 pp. 309-318
- acetic acid, benzyladenine, greenhouses, indole acetic acid, kinetin, mature plants, micropropagation, naphthaleneacetic acid, plantlets, roots, shoots
- A rapid micropropagation protocol for Nyctanthus arbor-tristis using nodal segments of the mature plant has been developed. The sterilized nodal segments were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg.L –¹ 6-benzylaminopurine (BAP). This medium combination regenerated 9.0 ± 0.17 shoots from each explant. The shoots were multiplied (41.0 ± 0.24 shoots per explant per culture vessel) by repeated subculturing of freshly induced shoots on MS medium at lower concentrations (1.0 mg.L –¹) each of BAP and Kinetin (Kn) and 0.5 mg.L –¹ indole-3 acetic acid (IAA). The shoots were rooted (17.0 ± 1.42 roots per shoot) in vitro on half-strength MS medium containing 3.0 mg.L –¹α -naphthalene acetic acid (NAA). The rooted plantlets hardened in the greenhouse with 89% rate of survival.