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Alpha-amylase Inhibition and Brine Shrimp Lethality Activities of Nine Medicinal Plant Extracts from South-West Nigerian Ethnomedicine

Author:
Ogbole, Omonike Oluyemisi, Aliu, Latifat Olabimpe, Abiodun, Oyindamola Olajumoke, Ajaiyeoba, Edith Oriabure
Source:
Journal of herbs, spices & medicinal plants 2016 v.22 no.4 pp. 319-326
ISSN:
1540-3580
Subject:
Acanthospermum hispidum, Alchornea, Artemia, Holarrhena floribunda, Ipomoea asarifolia, Nauclea diderrichii, Piper guineense, acarbose, alpha-amylase, butanol, ethyl acetate, inhibitory concentration 50, lethal concentration 50, medicinal plants, methanol, methylene chloride, mortality, plant extracts, solvents, toxicity, traditional medicine
Abstract:
Acanthospermum hispidum, Alchornea laxiflora, Calyptrochilum christyanum, Heliotropicum indicum, Holarrhena floribunda, Ipomoea asarifolia, Nauclea diderrichii, Piper guineense , and Tetracera scandens were extracted into methanol and screened for the availability of alpha-amylase inhibitors. Plant extracts’ toxicity was determined by brine shrimp lethality assay (BSLA). The most active plant, Nauclea diderichii , was fractionated into four solvent systems (n -hexane fractions, ethyl acetate, dichloromethane, and n -butanol) and tested. All the extracts showed varying degrees of alpha-amylase inhibitory activity. N. diderrichii with IC ₅₀ = 248.30 ± 0.27 µ g.mL –¹ had the highest inhibitory activity, while its butanol fraction with IC ₅₀ = 137.8 µ g.mL –¹ had activity comparable to the standard α -amylase inhibitor acarbose (IC ₅₀ = 177.50 ± 0.42 µ g.mL –¹). LC ₅₀ values for BSLA ranged from 11.35 to 1,127.50 µ g.mL –¹, with T. scandens being the most toxic with 11.35 µ g.mL –¹ and N. diderrichii the least toxic with 1,127.50 µ g.mL –¹.
Agid:
5592886