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Development of monoclonal antibodies specific for Ricinus agglutinins
- Brandon, David L., Hernlem, Bradley J.
- Food and agricultural immunology 2009 v.20 no.1 pp. 11
- Ricinus communis, agglutinins, cross reaction, enzyme-linked immunosorbent assay, food matrix, hybridomas, immunoglobulin G, immunoglobulin M, industrial byproducts, lubricants, monoclonal antibodies, plant extracts, poisoning, ricin, seeds, skim milk
- Ricin is a highly toxic, dichain ribosome-inactivating protein present in the seeds of Ricinus communis (castor), grown principally as a source of high quality industrial lubricant and as an ornamental. Because of its presence in industrial byproducts and its documented use for intentional poisoning, there is a need for analytical methodology to quantify ricin in both castor extracts and food matrices. We developed a panel of monoclonal antibodies to ricin, with most having strong cross-reactivity with RCA-1, a homologous but less toxic castor agglutinin. Some of the IgM-producing hybridomas appeared to produce a second IgG isotype and were further analyzed by fluorescence-activated cell sorting. The antibodies were effective in various ELISA formats, many with IC50’s in the range of 0.1-10 ng/mL and minimal matrix effects in skim milk. Assay specificity can be adjusted for analytical needs by varying the combination of antibodies in a sandwich ELISA format.