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Polyamines promote the development of embryonal-suspensor masses and the formation of somatic embryos in Picea glehnii

Author:
Nakagawa, Remi, Kurushima, Masahiro, Matsui, Masaaki, Nakamura, Ryouichi, Kubo, Takafumi, Funada, Ryo
Source:
In vitro cellular & developmental biology 2011 v.47 no.4 pp. 480-487
ISSN:
1054-5476
Subject:
Picea glehnii, light microscopy, putrescine, somatic embryogenesis, somatic embryos, spermidine
Abstract:
Effects of polyamines on the development of embryonal-suspensor masses (ESMs) and the formation of somatic embryos were examined in an effort to improve the frequency of somatic embryogenesis in Picea glehnii. Exogenous spermidine at 100 and 300 μM promoted the elongation of suspensors and the formation of somatic embryos. Exogenous putrescine at 100 μM increased the number of somatic embryos and decreased the time required for formation of somatic embryos. Thus, exogenous polyamines improved the maturation capacity of ESMs and promoted the formation of somatic embryos in P. glehnii. Light microscopy revealed that ESMs with high maturation capacity consisted of the three following cells: round or oval cells, elongated cells, and protean cells. These components might be related to the capacity for formation of somatic embryos.
Agid:
559432