PubAg

Main content area

Toxoplasma gondii microneme protein 8 (MIC8) is a potential vaccine candidate against toxoplasmosis

Author:
Liu, M. M., Yuan, Z. G., Peng, G. H., Zhou, D. H., He, X. H., Yan, C., Yin, C. C., He, Y., Lin, R. Q., Song, H. Q., Zhu, X. Q.
Source:
Parasitology research 2010 v.106 no.5 pp. 1079-1084
ISSN:
0932-0113
Subject:
DNA, Toxoplasma gondii, antibodies, cell-mediated immunity, immune response, immunization, immunoglobulin G, interferon-gamma, interleukin-10, interleukin-2, interleukin-4, mice, nucleotide sequences, toxoplasmosis, vaccines
Abstract:
Microneme protein 8 (MIC8) is considered a new essential invasion factor in Toxoplasma gondii. In the present study, a deoxyribonucleic acid vaccine expressing MIC8 of T. gondii was constructed and the immune response it induced in Kunming mice was evaluated. The gene sequence encoding MIC8 was inserted into the eukaryotic expression vector pVAX I, and the pVAX-MIC8 expression plasmid was constructed, and the plasmid diluted with PBS to l00 mg/100 µl was injected into the Kunming mice muscularly. Levels of IgG antibody, gamma-interferon (IFN-γ), interleukin-2 (IL-2), interleukin-4, and interleukin-10 were detected. The mice were challenged with tachyzoites of the virulent T. gondii RH strain at the 14th day after the last immunization to observe the survival time. The high level of IFN-γ, IL-2, and IgG antibody indicated that mice vaccinated with recombinant pVAX-MIC8 plasmid could elicit strong cellular and humoral immune responses and showed a significantly increased survival time (10.3 ± 0.9 days) compared with control mice which died within 5 days of challenge infection. These data demonstrate that the T. gondii MIC8 is a potential vaccine candidate against toxoplasmosis.
Agid:
559436