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Wall teichoic acid is an essential component of Staphylococcus aureus for the induction of human dendritic cell maturation

Hong, Sung Jun, Kim, Sun Kyung, Ko, Eun Byeol, Yun, Cheol-Heui, Han, Seung Hyun
Molecular Immunology 2017 v.81 pp. 135-142
Gram-positive bacteria, Staphylococcus aureus, T-lymphocytes, cell wall components, cell walls, cytokines, dendritic cells, humans, immunomodulation, inflammation, mutants, pathogens, phenotype, pneumonia, septic shock, teichoic acids
Staphylococcus aureus is a Gram-positive pathogen that can cause chronic skin inflammation, pneumonia, and septic shock. The immunomodulatory functions of wall teichoic acid (WTA), a glycopolymer abundantly expressed on the Gram-positive bacterial cell wall, are poorly understood. Here, we investigated the role of WTA in the phenotypic and functional activation of human monocyte-derived dendritic cells (DCs) treated with ethanol-killed S. aureus. WTA-deficient S. aureus mutant (ΔtagO) exhibited attenuated binding and internalization to DCs compared to the wild-type. ΔtagO induced lower expression of maturation markers on and cytokines in DCs than the wild-type S. aureus. Furthermore, autologous human peripheral blood mononuclear cells cocultured with ΔtagO-treated DCs exhibited a marked reduction in T cell proliferative activity, the expression of activation markers, and the production of cytokines compared to the wild-type S. aureus-stimulated DCs. Collectively, these results suggest that WTA is an important cell wall component of S. aureus for the induction of DC maturation and activation.