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Corydalis bungeana Turcz. attenuates LPS-induced inflammatory responses via the suppression of NF-κB signaling pathway in vitro and in vivo

Author:
Zhai, Xiao-Ting, Chen, Jia-Quan, Jiang, Cui-Hua, Song, Jie, Li, Dong-Yu, Zhang, Hao, Jia, Xiao-Bin, Tan, Wei, Wang, Shu-Xia, Yang, Yi, Zhu, Fen-Xia
Source:
Journal of ethnopharmacology 2016 v.194 pp. 153-161
ISSN:
0378-8741
Subject:
Corydalis, IKappaB kinase, Western blotting, anti-inflammatory activity, antibacterial properties, enzyme-linked immunosorbent assay, immunohistochemistry, inducible nitric oxide synthase, inflammation, interleukin-1beta, interleukin-6, liver, macrophages, mice, nitric oxide, phosphorylation, protein synthesis, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, secretion, signal transduction, traditional medicine, transcription factor NF-kappa B, tumor necrosis factor-alpha, China
Abstract:
Corydalis bungeana Turcz. (C. bungeana) is one of traditionally used medicines in China and possesses various biological effects, such as anti-inflammatory, antibacterial activity and inhibition of the immune function of the host.we studied the anti-inflammatory effect and molecular mechanism involved of C. bungeana both in vitro and in vivo model system in which the inflammatory response was induced by LPS treatment.Anti-inflammatory activity of C. bungeana was investigated by LPS-induced RAW 264.7 macrophages and BALB/c mice. The production and expression of pro-inflammatory cytokines were evaluated by Griess reagent, ELISA kits and RT-qPCR, respectively. Phosphorylation status of IκBα and p65 was illustrated by western blot assay.C. bungeana reduced the secretion of NO, TNF-α, IL-6 and IL-1β through inhibiting the protein expression of iNOS, TNF-α, IL-6 and IL-1β in vitro and in vivo. Western blot analysis suggested that C. bungeana supressed NF-κB activation via regulating the phosphorylation of IκBα and p65. Immunohistochemical assay also demostrated the histological inflammatory change in liver tissue.The results indicate that C. bungeana supresses the activation of NF-κB signaling pathway through inhibiting phosphorylation of IκBα and p65, which results in good anti-inflammatory effect. In addition, C. bungeana attenuates inflammatory reaction by supressing the expression of various inflammatory cytokines both in vivo and in vitro.
Agid:
5598262