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BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

Author:
Passarinho, Paul, Ketelaar, Tijs, Xing, Meiqing, van Arkel, Jeroen, Maliepaard, Chris, Hendriks, Mieke Weemen, Joosen, Ronny, Lammers, Michiel, Herdies, Lydia, den Boer, Bart, van der Geest, Lonneke, Boutilier, Kim
Source:
Plant molecular biology 2008 v.68 no.3 pp. 225-237
ISSN:
0167-4412
Subject:
Arabidopsis, Brassica napus, actin, biosynthesis, callus formation, cell growth, cell proliferation, cell walls, genes, microarray technology, microfilaments, phenotype, protein metabolism, seedlings, somatic embryogenesis, transcription (genetics), transcription factors
Abstract:
Ectopic expression of the Brassica napus BABY BOOM (BBM) AP2/ERF transcription factor is sufficient to induce spontaneous cell proliferation leading primarily to somatic embryogenesis, but also to organogenesis and callus formation. We used DNA microarray analysis in combination with a post-translationally regulated BBM:GR protein and cycloheximide to identify target genes that are directly activated by BBM expression in Arabidopsis seedlings. We show that BBM activated the expression of a largely uncharacterized set of genes encoding proteins with potential roles in transcription, cellular signaling, cell wall biosynthesis and targeted protein turnover. A number of the target genes have been shown to be expressed in meristems or to be involved in cell wall modifications associated with dividing/growing cells. One of the BBM target genes encodes an ADF/cofilin protein, ACTIN DEPOLYMERIZING FACTOR9 (ADF9). The consequences of BBM:GR activation on the actin cytoskeleton were followed using the GFP:FIMBRIN ACTIN BINDING DOMAIN2 (GFP:FABD) actin marker. Dexamethasone-mediated BBM:GR activation induced dramatic changes in actin organization resulting in the formation of dense actin networks with high turnover rates, a phenotype that is consistent with cells that are rapidly undergoing cytoplasmic reorganization. Together the data suggest that the BBM transcription factor activates a complex network of developmental pathways associated with cell proliferation and growth.
Agid:
560113