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Head Capsule Width is Useful for Determining Larval Instar in Heilipus lauri (Coleoptera: Curculionidae)

Castañeda-Vildózola, Álvaro, González-Hernández, Héctor, Equihua-Martínez, Armando, Valdez-Carrasco, Jorge, Peña, Jorge E., Cazado, Lucas Emiliano, Franco-Mora, Omar
The Florida entomologist 2016 v.99 no.4 pp. 822-825
Curculionidae, digital images, geometry, head, instars, larvae, linear models, rearing
The purpose of this study was to determine the number of larval instars for Heilipus lauri Boheman (Coleoptera: Curculionidae). We reared 385 larvae under laboratory conditions and measured the width of the larval head capsules from digital images. Data were analyzed using the Hcap program and Dyar's rule. Analysis of the frequency distribution showed the existence of 4 distinct peaks. The mean Dyar's constant was 1.42, where the relationship between the natural logarithm of the head capsule widths and the number of larval instars resulted in a near-perfect geometric growth for each instar. The excellent fit of the data to a linear model suggests that there was nearly no overlap among the 4 instars in the head capsule measurements, making it possible to distinguish the 4 instars of H. lauri accurately.