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I: Biomarker quantification in fish exposed to crude oil as input to species sensitivity distributions and threshold values for environmental monitoring

Author:
Sanni, Steinar, Björkblom, Carina, Jonsson, Henrik, Godal, Brit F., Liewenborg, Birgitta, Lyng, Emily, Pampanin, Daniela M.
Source:
Marine environmental research 2017 v.125 pp. 10-24
ISSN:
0141-1136
Subject:
DNA adducts, biomarkers, environmental monitoring, genotoxicity, halibut, histopathology, immunotoxicity, metabolites, oxidative stress, petroleum, polycyclic aromatic hydrocarbons, risk assessment, salmon, turbot
Abstract:
The aim of this study was to determine a suitable set of biomarker based methods for environmental monitoring in sub-arctic and temperate offshore areas using scientific knowledge on the sensitivity of fish species to dispersed crude oil. Threshold values for environmental monitoring and risk assessment were obtained based on a quantitative comparison of biomarker responses. Turbot, halibut, salmon and sprat were exposed for up to 8 weeks to five different sub-lethal concentrations of dispersed crude oil. Biomarkers assessing PAH metabolites, oxidative stress, detoxification system I activity, genotoxicity, immunotoxicity, endocrine disruption, general cellular stress and histological changes were measured. Results showed that PAH metabolites, CYP1A/EROD, DNA adducts and histopathology rendered the most robust results across the different fish species, both in terms of sensitivity and dose-responsiveness. The reported results contributed to forming links between biomonitoring and risk assessment procedures by using biomarker species sensitivity distributions.
Agid:
5603213