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The putative serine protease inhibitor (serpin) genes encoded on Echinococcus multilocularis genome and their expressions in metacestodal stage

Author:
Sasaki, Mizuki, Sako, Yasuhito
Source:
Veterinary parasitology 2017 v.233 pp. 20-24
ISSN:
0304-4017
Subject:
Echinococcus multilocularis, Western blotting, genes, metacestodes, parasites, polyclonal antibodies, proteinase inhibitors, proteins, serine proteinases, staining
Abstract:
Two putative serpin genes were identified in Echinococcus multilocularis, in addition to the already reported serpinEmu, and were designated as serpin2Emu and serpin3Emu. Western blot analysis using polyclonal antibodies against serpinEmu, putative serpin2Emu protein, and putative serpin3Emu protein indicated that all three proteins were localized in both intracellular and excretory-secretory (ES) fractions of E. multilocularis metacestodes. In addition, immune staining of parasite tissue indicated that all three proteins were localized at the germinal layer.
Agid:
5604245