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Prediction of lymph node parasite load from clinical data in dogs with leishmaniasis: An application of radial basis artificial neural networks

Author:
Torrecilha, Rafaela Beatriz Pintor, Utsunomiya, Yuri Tani, Batista, Luís Fábio da Silva, Bosco, Anelise Maria, Nunes, Cáris Maroni, Ciarlini, Paulo César, Laurenti, Márcia Dalastra
Source:
Veterinary parasitology 2017 v.234 pp. 13-18
ISSN:
0304-4017
Subject:
Leishmania infantum, artificial intelligence, biomarkers, diagnostic techniques, dog diseases, dogs, immunologic techniques, inflation, infrastructure, leishmaniasis, lymph nodes, monitoring, neural networks, parasite load, parasites, prediction, public services and goods, quantitative polymerase chain reaction, therapeutics
Abstract:
Quantification of Leishmania infantum load via real-time quantitative polymerase chain reaction (qPCR) in lymph node aspirates is an accurate tool for diagnostics, surveillance and therapeutics follow-up in dogs with leishmaniasis. However, qPCR requires infrastructure and technical training that is not always available commercially or in public services. Here, we used a machine learning technique, namely Radial Basis Artificial Neural Network, to assess whether parasite load could be learned from clinical data (serological test, biochemical markers and physical signs). By comparing 18 different combinations of input clinical data, we found that parasite load can be accurately predicted using a relatively small reference set of 35 naturally infected dogs and 20 controls. In the best case scenario (use of all clinical data), predictions presented no bias or inflation and an accuracy (i.e., correlation between true and predicted values) of 0.869, corresponding to an average error of ±38.2 parasites per unit of volume. We conclude that reasonable estimates of L. infantum load from lymph node aspirates can be obtained from clinical records when qPCR services are not available.
Agid:
5604252