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Four new steroids from the endophytic fungus Chaetomium sp. M453 derived of Chinese herbal medicine Huperzia serrata

Author:
Yu, Fei-Xue, Li, Zhe, Chen, Yao, Yang, Yin-He, Li, Guo-Hong, Zhao, Pei-Ji
Source:
Fitoterapia 2017 v.117 pp. 41-46
ISSN:
0367-326X
Subject:
Chaetomium, Huperzia serrata, X-ray diffraction, acetylcholinesterase, endophytes, fermented foods, fungi, herbal medicines, nuclear magnetic resonance spectroscopy, spectral analysis, steroids
Abstract:
An endophytic fungus, Chaetomium sp. M453, was isolated from Huperzia serrata (Thunb. ex Murray) Trev. and subjected to phytochemical investigation. Three unusual C25 steroids, neocyclocitrinols E-G (1–3), and 3β-hydroxy-5,9-epoxy-(22E,24R)-ergosta-7,22-dien-6-one (4) together with three known steroids were isolated from solid fermentation products of the fungus, which were elucidated by extensive spectroscopic analyses, including 1D-, 2D-NMR, and HR-ESI-MS experiments. The absolute configuration of 1 was determined by X-ray crystallographic analysis and CD analyses. The acetylcholinesterase inhibitory activities of compounds 1–4 were tested in vitro. Compound 4 showed weak acetylcholinesterase inhibitory activity.
Agid:
5604589