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Antimicrobial effects of Lactobacillus plantarum and Lactobacillus acidophilus against multidrug-resistant enteroaggregative Escherichia coli

Author:
Kumar, Manesh, Dhaka, Pankaj, Vijay, Deepthi, Vergis, Jess, Mohan, Vysakh, Kumar, Ashok, Kurkure, Nitin V., Barbuddhe, Sukhadeo B., Malik, S.V.S., Rawool, Deepak B.
Source:
International journal of antimicrobial agents 2016 v.48 no.3 pp. 265-270
ISSN:
0924-8579
Subject:
Escherichia coli, Lactobacillus acidophilus, Lactobacillus plantarum, anti-infective agents, antibacterial properties, coculture, colon, feces, histopathology, ileum, in vitro studies, in vivo studies, mice, multiple drug resistance, plate count, probiotics, quantitative polymerase chain reaction, therapeutics
Abstract:
The in vitro and in vivo antimicrobial effects of Lactobacillus plantarum and Lactobacillus acidophilus were evaluated individually and synergistically against multidrug-resistant enteroaggregative Escherichia coli (MDR-EAEC). In vitro evaluation of each probiotic strain when co-cultured with MDR-EAEC isolates revealed a reduction in MDR-EAEC counts (eosin–methylene blue agar) in a dose- and time-dependent manner: probiotics at a dose rate of 10¹⁰ CFU inhibited MDR-EAEC isolates at 72 h post-inoculation (PI), whereas at lower concentrations (10⁸ and 10⁹ CFU) MDR-EAEC isolates were inhibited at 96 h PI. The synergistic antimicrobial effect of both probiotic strains (each at 10¹⁰ CFU) was highly significant (P < 0.01) and inhibited the growth of MDR-EAEC isolates at 24 h PI. For in vivo evaluation, weaned mice were fed orally with 10⁷ CFU of MDR-EAEC. At Day 3 post-infection, treated mice were fed orally with the probiotic strains (each at 10¹⁰ CFU). Compared with the control, post-treatment a significant (P < 0.01) reduction in MDR-EAEC counts was observed in faeces by Day 2 and in intestinal tissues of treated mice by Days 3 and 4 as evidenced by plate count (mean 2.71 log and 2.27 log, respectively) and real-time PCR (mean 1.62 log and 1.57 log, respectively) methods. Histopathologically, comparatively mild changes were observed in the ileum and colon from Days 3 to 5 post-treatment with probiotics; however, from Day 6 the changes were regenerative or normal. These observations suggest that these probiotic strains can serve as alternative therapeutics against MDR-EAEC-associated infections in humans and animals.
Agid:
5605018